Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions

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Abstract
Antisera to sodium dodecyl sulfate-derived polyoma virion polypeptides was produced [in rabbits]. Using this antisera, cytoplasmic synthesis of V1, V2 and V3 polypeptides at 18 h postinfection and subsequent transport to the nucleus by 22 h postinfection were detected by immunofluorescence. Anti-V1, anti-V2 and anti-V3 sera did not react with intact virions in an immunodiffusion assay, nor did they possess hemagglutination inhibition or viral neutralization activity. Antiserum produced against the 4 host histone polypeptides (V4 through V7) demonstrated immunofluorescence when reacted wtih polyoma-infected [mouse embryo and baby mouse kidney] cells but not with uninfected cells. Anti-histone serum was also capable of neutralizing viral infectivity, inhibiting hemagglutination and reacting with whole virions in an immunodiffusion assay.