Cloning and expression of the lysostaphin gene in Bacillus subtilis and Lactobadllus casei
Open Access
- 1 March 1992
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 14 (3) , 72-76
- https://doi.org/10.1111/j.1472-765x.1992.tb00651.x
Abstract
The lysostaphin structural gene was cloned in Bacillus subtilis DSM402 and in Lactobacillus casei 102S. The gene was expressed in both organisms and active lysostaphin was released into the medium. Lysostaphin produced by these organisms induced lysis of growing and heat inactivated staphylococci. Expression in a protective starter organism is a prerequisite to produce lysostaphin in situ in fermenting foods and hence, to reduce the hygienical risk of staphylococcal food poisoning.Keywords
This publication has 15 references indexed in Scilit:
- Structurally stable Bacillus subtilis cloning vectorsGene, 1990
- High efficiency transformation of E.coli by high voltage electroporationNucleic Acids Research, 1988
- The molecular organization of the lysostaphin gene and its sequences repeated in tandemMolecular Genetics and Genomics, 1987
- Transformation ofLactobacillus caseiby electroporationFEMS Microbiology Letters, 1987
- Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans.Proceedings of the National Academy of Sciences, 1987
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- Studies on LysostaphinEuropean Journal of Biochemistry, 1973
- LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUSProceedings of the National Academy of Sciences, 1964
- A MEDIUM FOR THE CULTIVATION OF LACTOBACILLIJournal of Applied Bacteriology, 1960
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958