Localization of Lipoxygenases 1 and 2 in Germinating Soybean Seeds by an Indirect Immunofluorescence Technique

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Abstract
The effect of CN− and N2 on the electrical membrane potential (Em) was compared with that of CN− on the ATP levels in cotyledons of Gossypium hirsutum and in Lemna gibba L. In mature cotton tissue, CN− depolarized Em to the energy-independent diffusion potential (ED) in the dark. In the light Em recovered transiently. The same was observed in leaves of Nicotiana, Avena, Impatiens, Kalanchoë, and in Lemna. In contrast, in young cotton cotyledons and tobacco leaves and, to a large extent, in +sucrose-grown Lemna, Em was depolarized to ED also in the light in a similar way as in the dark.