Confounding of Alternate Respiration by Lipoxygenase Activity

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Abstract
The initital burst of respiratory activity (QO2) of imbibing soybean (Glycine max [L.] Merr. ''Wayne'') seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O2 consumption is also highly sensitive to propyl gallate. Soybean lipoxygenase exhibits similar characteristics of insensitivity to cyanide and sensitivity to salicylhydroxamate and to propyl gallate. The initial burst of respiration is enhanced by the addition of linoleic acid, a lipoxygenase substrate. Apparently the conventional tests for alternate respiration in plant tissues can be confounded by lipoxygenase. Perhaps propyl gallate can be used to assess the possible participation of lipoxygenase in the O2 uptake by plant tissues.