Estimation of membrane potentials of individual lymphocytes by flow cytometry.

1 November 1979

journal article
research article
Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences

Vol. 76 (11) , 5728-5730
https://doi.org/10.1073/pnas.76.11.5728

Abstract

The membrane potentials of individual cells could be estimated by flow cytometric quantitation of the cells'' uptake of the fluorescent lipophilic cationic dye 3,3''-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients were uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarized only a fraction of the lymphocytes. The flow cytometric technique allowed precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.

Keywords

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