The Effects Of Serum From Women With Miscarriages On The In Vitro Development Of Mouse Preimplantation Embryos

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Abstract
It is generally believed that some human miscarriages result from embryotoxic factors existing in the sera. To study the embryotoxicity of such sera, 3.5-day-old mouse blastocysts were cultured for 72 h on 80% sera from different groups of women. After 72 h there was no blastocystic development in 53.2% of the cases grown on sera from women after two or more miscarriages, and none in 33.6% of the blastocysts grown on sera from women after one miscarriage, as compared with 8.2% and 12% respectively on control sera. Sera from women with miscarriages were divided into ‘high risk’ (50% or more embryotoxicity) and ‘low risk’ (less than 50% embryotoxicity) sera. The ‘high risk’ sera from two or more miscarriages caused an average of 72.1% undevelopment, while the ‘low risk’ sera (less than 50% embryotoxicity) from the same group caused 33.6% undevelopment. The ‘high risk’ sera from one miscarriage were embryotoxic to 55.8% of the blastocysts and the ‘low risk’ sera from the same group caused only 8.7% undevelopment. No significant differences were found in the mean serum concentrations of folic acid, zinc and copper of many of the experimental groups, in comparison with controls. The embryotoxic factor/s which exist in the ‘high risk’ sera from women with miscarriages are still not known.
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