Tetrahymena : Effect of Freezing and Subsequent Thawing on Breeding Performance

Abstract
Tetrahymena pyriformis , syngen 1, frozen in 10 percent dimethylsulfoxide, stored for 2 months, and then thawed could conjugate normally. More significantly, they were viable, in normal numbers, through two sexual reorganizations. The strains apparently did not sustain genetic damage during the treatment. The techniques offer considerable promise for the maintenance of breeding stocks in ciliated protozoans.
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