Glucose transporters in isolated chromaffin cells. Effects of insulin and secretagogues

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Abstract
1. Isolated chromaffin cells from bovine adrenal medulla were used to study glucose transport in a homogeneous neural tissue. 2. The affinity of glucose transporters was 1.20 .+-. 0.52 mM by the infinite-cis technique and 1.02 .+-. 0.09 mM by the direct transport experiments. 3. The affinity for 2-deoxyglucose of these transporters was 2.3 mM. 4. The glucose transporters, quantified by [3H]cytochalasin B binding, were 419532 .+-. 120740 receptors/cell, which corresponds to about 7.2 .+-. 2 pmol/mg of protein, with KD = 0.1 .mu.M. 5. High-affinity insulin receptors with KD = 3.95 nM were present at a density of 68,400 .+-. 7,500 per cell. 6. Insulin and secretagogues increased glucose transport, raising the transporter number at the plasma membrane without changes in the affinity.