Influence of embedding media in prolactin labelling with immunogold techniques
- 1 August 1986
- journal article
- conference paper
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 18 (8) , 429-433
- https://doi.org/10.1007/bf01675335
Abstract
Immunogold labelling of prolactin in three different embedding media was compared. The polymeric prolactin in secretory granules was labelled in the three media, however, acrylic monomers (Lowicryl K4M and LR White) provided a more intense labelling with higher dilutions of the primary antibody, compared to the labelling in the epoxy resin (araldite). An intense labelling of monomeric prolactin in Golgi complex was detected only in acrylic embedments, and the labelling on the rough endoplasmic reticulum was significant only in LR White embedded tissues.This publication has 12 references indexed in Scilit:
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