Multiple Activities Of DNA Polymerase from Visna Virus
- 1 January 1973
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 3 (6) , 525-539
- https://doi.org/10.1080/00327487308061535
Abstract
Daoxyribonucleic acid (DNA) polymerase Isolated from visne virus has been separated into three enzymatically active polypeptides by column chromatography on DEAE-cellulose. They are designated polymerase I, II and III according to the order of elutlon from the column. The three enzymes are different in their pH optimum, sensitivity to N-ethylmaleimide, rate of catalytic reaction, and preference of template. The molecular weights were estimated to be 125, 000 for polymerase I and 18, 000 for II.Keywords
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