Block in Nuclear Localization of period Protein by a Second Clock Mutation, timeless
- 18 March 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 263 (5153) , 1606-1609
- https://doi.org/10.1126/science.8128247
Abstract
In wild-type Drosophila, the period protein (PER) is found in nuclei of the eyes and brain, and PER immunoreactivity oscillates with a circadian rhythm. The studies described here indicate that the nuclear localization of PER is blocked by timeless (tim), a second chromosome mutation that, like per null mutations, abolishes circadian rhythms. PER fusion proteins without a conserved domain (PAS) and some flanking sequences are nuclear in tim mutants. This suggests that a segment of PER inhibits nuclear localization in tim mutants. The tim gene may have a role in establishing rhythms of PER abundance and nuclear localization in wild-type flies.Keywords
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