Inheritance of RAPD Markers in the Guppy Fish, Poecilia reticulata

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Abstract
The recent method of revealing DNA-based polymorphisms by Random Amplified Polymorphic DNA (RAPD) fingerprinting, which involves PCR amplification of genomic DNA using a single primer of arbitrary nucleotide sequence, was reported in 1990 [20, 24]. Since then the RAPD method has been applied to a wide range of organisms and its advantages over RFLP fingerprinting method have been extensively reviewed [1–3, 10, 12, 14, 17, 20, 22, 24, 26]. However, there are few reports on RAPD fingerprinting of fishes. RAPD fingerprinting has been used by Dinesh et al. [5 6–7] for detection of DNA polymorphisms in some fish species including color varieties of the guppy (Poecilia reticulata) and tiger barb (Barbus tetrazona). Kubota et al. [15] used this method to detect radiation-induced DNA damages in the medaka, Oryzias latipes. Recently, Johnson et al. [13] identified 721 RAPD polymorphisms between two laboratory strains of the zebrafish, Danio rerio, and of these, 401 of them were used to construct a genetic linkage map [19]. The present study so far is the only one which demonstrates Mendelian inheritance of RAPD markers in fish.