USE OF POROUS CELLULOSE ESTER MEMBRANES IN THE PRIMARY ISOLATION AND SIZE DETERMINATION OF PLEUROPNEUMONIA-LIKE ORGANISMS

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Abstract
Morowitz, Harold J. (Yale University, New Haven, Conn.), Mark E. Tourtellotte, and Mary E. Pollack . Use of porous cellulose ester membranes in the primary isolation and size determination of pleuropneumonia-like organisms. J. Bacteriol. 85: 134–136. 1963.—Millipore filters were used in the primary isolation and sizing of a number of strains of pleuropneumonia-like organisms (PPLO). Maximal pore diameters were checked by filtration of viruses of known sizes. All the PPLO strains studied produced cells capable of passing through a 0.22-μ filter. A number of strains produced forms which could be titered after filtration through a 0.1-μ filter. A number of primary isolations were made from filtrates.