Antibody responses in patients with farmer's lung disease to antigens fromThermoactinomyces vulgaris
- 1 February 1975
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 74 (1) , 35-41
- https://doi.org/10.1017/s0022172400046696
Abstract
A serological analysis of mycelial antigens ofThermoactinomyces vulgarisin immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid.Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies toT. vulgaris;the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.Keywords
This publication has 11 references indexed in Scilit:
- Antibody responses in patients with farmer's lung disease to antigens fromMicropolyspora faeniEpidemiology and Infection, 1974
- Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens.1973
- Characterization of thermophilic actinomycetes isolated from residential heating and humidification systemsJournal of Allergy and Clinical Immunology, 1973
- Micropolysporafaeniand farmer's lung diseaseEpidemiology and Infection, 1973
- Hypersensitivity Pneumonitis: Experimental Production in Guinea Pigs with Antigens of Micropolyspora faeniPathobiology, 1973
- Immunofluorescent studies in patients with farmer's lungJournal of Allergy and Clinical Immunology, 1971
- A colorimetric estimation of lipopolysaccharidesFEBS Letters, 1971
- The extracellular antigens of Micropolyspora faeni: their significance in farmer's lung diseaseEpidemiology and Infection, 1970
- Farmer's Lung in an Adolescent BoyArchives of Pediatrics & Adolescent Medicine, 1969
- Precipitin (F.L.H.) Test in Farmer's LungThorax, 1965