Neural-tube defects caused by 5-bromodeoxyuridine in chicks

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Abstract
Chick embryos were explanted at HH stage 4 and cultured for 24 h on thin albumen with 10 μg/ml 5‐bromodeoxyuridine (BrdU). BrdU inhibited closure of the neural tube in over 90% of the embryos. Mitotic figures were found throughout adversely effected neuroepithelium, suggesting that interkinetic nuclear migration had been inhibited. Electron microscopic studies of BrdU‐treated neural‐tube cells showed fewer and more amorphous cytoplasmic extensions, microfilaments, and desmosomal (functional) tonofibrils than in controls, but micro‐tubules were unaffected.