Improved ultrastructural preservation of yeast cells for scanning electron microscopy
- 1 November 1996
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 184 (2) , 81-87
- https://doi.org/10.1111/j.1365-2818.1996.tb00002.x
Abstract
The processing of yeast cells for scanning electron microscopy by conventional sequential fixation with glutaralde-hyde and osmium tetroxide and subsequent dehydration and critical point-drying caused pronounced deformation and visible shrinkage in all basidiomycetous and ascomy-cetous yeast strains studied. The mean cell diameter decreased to nearly 60 and 70%, respectively. After an additional sequential fixation with 1% tannic acid and 0–5% uranyl acetate the cell shrinkage was significantly reduced, but the most important result was a considerable reduction of wrinkling and deformation of the yeast cells.Keywords
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