Infection of One-Day-Old Fertile Hen's Egg with Herpes Simplex Virus

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Abstract
Summary: A method for the inoculation of herpes simplex virus into the blastoderm of 1-day-old fertile hen's egg is described. After infection, embryonic development ceases, but an increment of virus titer occurs in the egg white of such nondeveloping eggs. Infection can be achieved by as little as one infectious unit as judged by titration on the CAM of 13-day-old embryos. Since the appearance of virus in the egg white was irregular, due to the inhomogeneity of the material, this was replaced by yolk saline. With the eggs thus treated, growth curves were obtained, and it could be demonstrated that newly formed virus was released into the yolk saline at an increasingly higher rate with the passage of time up to 5 days following inoculation, while the whole egg titer, presumably representing intracellular virus, showed a sharp rise already at the end of 24 hr. Practical applications of the techniques are discussed.