A THIN-LAYER CHROMATOGRAPHIC STUDY OF SOME AMPHIPLOIDS IN AVENA

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Abstract
A thin-layer chromatographic study was carried out using five synthetic Avena amphiploids and their parental genotypes. The Dass-Nybom solvent and an extraction time of 120 hr were found to be optimum for Avena. About 42% of 3 total of 62 spots were common to all, or all but one species, and these were considered characteristic for the genus. The over-all additiveness of the parental spectra was low (32.5%) in the experimental alloploids; therefore, comparisons of total spectra of natural alloploids and their putative progenitors may not be useful in Avena. A number of hybrid spots, due to complementary gene interactions, was detected. Selected spots specific for either the A- or C-genome diploids could be traced in the polyploids. These suggested the presence of the C genome in the new tetraploid species A. magna, and in the hexaploids, and confirmed A. magna as the putative tetraploid ancestor of the hexaploid species. The chromatographic phenotype, due to its large number of well defined traits, may be very useful in studying gene interactions in polyploid Avena and for marking linkage groups in chromosome deficient lines.