Optimal design and validation of antiviral siRNA for targeting HIV-1
Open Access
- 1 January 2007
- journal article
- research article
- Published by Springer Nature in Retrovirology
- Vol. 4 (1) , 80
- https://doi.org/10.1186/1742-4690-4-80
Abstract
We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains.Keywords
This publication has 19 references indexed in Scilit:
- siVirus: web-based antiviral siRNA design software for highly divergent viral sequencesNucleic Acids Research, 2006
- A novel approach for inhibition of HIV-1 by RNA interference: counteracting viral escape with a second generation of siRNAs2005
- Antiviral RNAi therapy: emerging approaches for hitting a moving targetGene Therapy, 2005
- Molecular strategies to inhibit HIV-1 replicationRetrovirology, 2005
- HIV-1 can escape from RNA interference by evolving an alternative structure in its RNA genomeNucleic Acids Research, 2005
- Unlocking the potential of the human genome with RNA interferenceNature, 2004
- Human Immunodeficiency Virus Type 1 Escapes from RNA Interference-Mediated InhibitionJournal of Virology, 2004
- Human Immunodeficiency Virus Type 1 Escape from RNA InterferenceJournal of Virology, 2003
- Initiation of HIV-1 Reverse Transcription Is Regulated by a Primer Activation SignalJournal of Biological Chemistry, 2001
- Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegansNature, 1998