INVITRO CHEMOTHERAPY TESTING OF TRANSITIONAL CELL-CARCINOMA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 17  (5) , 395-400
Abstract
The chemotherapeutic drug sensitivity of live cells freshly isolated from 2 human bladder tumors was studied. Substantial differences in the sensitivities of the 2 tumors to various chemotherapeutic drugs and different sensitivities of a given tumor to different to different drugs were found. Such data could be valuable clinically. The therapeutic value of cyclophosphamide, thiotepa and methotrexate was questionable for these 2 tumors because an increase in thymidine incorporation was seen after treatment with these drugs. A similar increase was observed after treatment with 5-fluorouracil in cells of a tissue-culture cell line derived from a human bladder tumor. Pretreatment of the cells with 5-fluorouracil reduced this effect. After 12-18 h exposure to 5-fluorouracil, the cells formed a confluent monolayer within 9 days. A similar treatment with doxorubicin hydrochloride resulted in detachment of the cells and formation of aggregates. The aggregated cells excluded trypan blue for at least 9 days after the doxorubicin hydrochloride was removed.