Specificity of the Break Produced by Restricting Endonuclease R 1 in Simian Virus 40 DNA, as Revealed by Partial Denaturation Mapping
- 1 November 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11) , 3215-3219
- https://doi.org/10.1073/pnas.69.11.3215
Abstract
Superhelical circular (form I) SV40 DNA was converted to linear molecules by the action of a partially purified restriction enzyme of Resistance Transfer Factor-R(1) of Escherichia coli. The resulting linear DNA molecules are full length, as judged by their sedimentation through alkaline sucrose gradient and by direct observation in an electron microscope. Nicked circular (form II) DNA was found as an intermediate in the conversion of form I DNA to linear DNA. Analysis of partial denaturation maps obtained by alkaline denaturation of the unitlength linear molecules showed that the break in SV40 DNA occurred at a specific site on the DNA.Keywords
This publication has 9 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Cleavage of Simian Virus 40 DNA at a Unique Site by a Bacterial Restriction EnzymeProceedings of the National Academy of Sciences, 1972
- Characterization by electron microscopy of the complex formed between T4 bacteriophage gene 32-protein and DNAJournal of Molecular Biology, 1972
- Sequence Heterogeneity in Closed Simian Virus 40 Deoxyribonucleic AcidJournal of Virology, 1972
- Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzaeProceedings of the National Academy of Sciences, 1971
- T4 Bacteriophage Gene 32: A Structural Protein in the Replication and Recombination of DNANature, 1970
- A Restriction enzyme from Hemophilus influenzaeJournal of Molecular Biology, 1970
- Partial denaturation of thymine- and 5-bromouracil-containing λ DNA in alkaliJournal of Molecular Biology, 1970
- Heterogeneous DNof simian virus 40.1969