An Evaluation of Some Aniline Dyes as Nuclear Stains in Trichrome Procedures

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Abstract
A comparison of the utility of gallocyanin, gallamine blue, celestine blue B and acid alizarin blue BB as nuclear stains for material to be counterstained by the van Gieson, Kornhauser, Gomori and Masson technics has been made. With all these nuclear stains except gallocyanin there were definite deficiencies. However, gallocyanin routinely has given excellent results and can be highly recommended. This dye is not specific for nucleic acids but rather stains some material with much the same spatial distribution in the cell as the nucleic acids.