Mutational Loss of a K + and NH 4 + Transporter Affects the Growth and Endospore Formation of Alkaliphilic Bacillus pseudofirmus OF4

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Abstract
A putative transport protein (Orf9) of alkaliphilic Bacillus pseudofirmus OF4 belongs to a transporter family (CPA-2) of diverse K + efflux proteins and cation antiporters. Orf9 greatly increased the concentration of K + required for growth of a K + uptake mutant of Escherichia coli . The cytoplasmic K + content of the cells was reduced, consistent with an efflux mechanism. Orf9-dependent translocation of K + in E. coli is apparently bidirectional, since ammonium-sensitive uptake of K + could be shown in K + -depleted cells. The upstream gene product Orf8 has sequence similarity to a subdomain of KTN proteins that are associated with potassium-translocating channels and transporters; Orf8 modulated the transport capacities of Orf9. No Orf9-dependent K + (Na + )/H + antiport activity was found in membrane vesicles. Nonpolar deletion mutants in the orf9 locus of the alkaliphile chromosome exhibited no K + -related phenotype but showed profound phenotypes in medium containing high levels of amine-nitrogen. Their patterns of growth and ammonium content suggested a physiological role for the orf9 locus in bidirectional ammonium transport. Orf9-dependent ammonium uptake was observed in right-side-out membrane vesicles of the alkaliphile wild type and the mutant with an orf8 deletion. Uptake was proton motive force dependent and was inhibited by K + . Orf9 is proposed to be designated AmhT (ammonium homeostasis). Ammonium homeostasis is important in high-amine-nitrogen settings and is particularly crucial at high pH since cytosolic ammonium accumulation interferes with cytoplasmic pH regulation. Endospore formation in amino-acid-rich medium was significantly defective and germination was modestly defective in the orf9 and orf7 - orf10 deletion mutants.