IMMUNOCHEMICAL STUDY ON BASEMENT-MEMBRANE (TYPE-IV) COLLAGENS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (1) , 109-116
Abstract
Basement membrane (type IV) collagens were extracted from a mouse tumor with acetic acid and from human placenta after limited enzymatic digestion. Antisera were produced against collagens in rabbits and guinea pigs, and examined by various assays. These antisera were specific for basement membrane collagen and showed little or no cross-reactions with interstitial collagens, types I, II and III or with human placenta collagen consisting of .alpha.A and .beta.B chains. Varying cross-reaction degrees were observed between antisera to human and mouse type IV collagen. Immunochemical analyses demonstrated presence of 3 distinct determinants in the tumor type IV collagen. Rabbit antisera against this antigen reacted with collagenase-resistant segments or with a collagenous, disulfide-bonded segment (P3). Guinea pig antisera recognized primarily antigenic determinants in the P3 segment. Antisera to placenta type IV collagen reacted with another collagenous, pepsin fragment (P1) which lacks disulfide bonds. These antisera showed complete cross-reaction with collagenous .alpha.1(IV) chains prepared from pepsin digests of human placenta and bovine lens capsule.