NUCLEOTIDES IN BEER

Abstract
Nucleotidic material was isolated by anion exchange chromatography of beer on a Dowex 1 × 8 (formate) column. Ultraviolet spectroscopy and retention volume data suggested the presence of cytidine monophosphate (CMP) [max. 5 mg/litre], in one of the fractions. No other mononucleotides were detected. Chromatography of charcoal extracts of beer on DEAE Sephadex A25 failed to yield a fraction containing mononucleotides. Triangular taste tests indicated that addition of adenosine-5′-monophosphate (AMP) and guanosine-5′-monophosphate (GMP) to beer (30 mg/litre and 25 mg/litre respectively) did not cause significant flavour changes.

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