Structure and function of a transcriptional network activated by the MAPK Hog1

Publisher Website
Google Scholar
Abstract
Erin O'Shea and colleagues present a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast derived from gene expression analyses in single- and multiple-mutant strains. The network reveals interactions involved in signal integration and processing and could serve as model for investigations into other gene regulatory networks. Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.