Transcription of Clostridium cellulovorans Cellulosomal Cellulase and Hemicellulase Genes
Open Access
- 15 April 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (8) , 2520-2527
- https://doi.org/10.1128/jb.185.8.2520-2527.2003
Abstract
Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. Northern hybridizations revealed that the cellulosomal cbpA gene cluster is transcribed as polycistronic mRNAs of 8 and 12 kb. The 8-kb mRNA coded for cbpA and exgS, and the 12-kb mRNA coded for cbpA, exgS, engH, and engK. The sizes of the mRNAs were about 3 kb for engE, 1.8 kb for manA, 2.7 kb for xynA, and 4 kb for pelA, indicating monocistronic transcription of these genes. Primer extension and S1 nuclease analysis of C. cellulovorans RNA showed that the transcriptional start sites of cbpA, engE, manA, and hbpA were located 233, 97, 64, and 61 bp upstream from the first nucleotide of each of the respective translation initiation codons. Alignment of the cbpA, engE, manA, and hbpA promoter regions provided evidence for highly conserved sequences that exhibited strong similarity to the σA consensus promoter sequences of gram-positive bacteria.Keywords
This publication has 39 references indexed in Scilit:
- Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellumApplied and Environmental Microbiology, 2002
- An Antisense RNA-Mediated Transcriptional Attenuation Mechanism Functions inEscherichia coliJournal of Bacteriology, 2002
- α-Galactosidase Aga27A, an Enzymatic Component of theClostridium josuiCellulosomeJournal of Bacteriology, 2002
- Control of cellulase formation by trehalose in Clostridium papyrosolvens CFR-703Process Biochemistry, 2001
- Properties and Mutation Analysis of the CelK Cellulose-Binding Domain from the Clostridium thermocellum CellulosomeJournal of Bacteriology, 2001
- Transcriptional analysis of theClostridium cellulovoransendoglucanase gene,engBFEMS Microbiology Letters, 1994
- Promoter recognition by Escherichia coli RNA polymerase. Effects of single base pair deletions and insertions in the spacer DNA separating the -10 and -35 regions are dependent on spacer DNA sequenceBiochemistry, 1993
- The interaction between Bacillus subtilis sigma-A (σA) factor and RNA polymerase with promotersBiochimie, 1992
- Recent advances in the genetics of the clostridiaFEMS Microbiology Letters, 1989
- Promoter recognition by Escherichia coli RNA polymeraseJournal of Molecular Biology, 1989