α-Galactosidase Aga27A, an Enzymatic Component of theClostridium josuiCellulosome
Open Access
- 15 January 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (2) , 600-604
- https://doi.org/10.1128/jb.184.2.600-604.2002
Abstract
TheClostridium josui aga27Agene encodes the cellulosomal α-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various α-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A α-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.Keywords
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