EFFECT OF BLEOMYCIN ON MOUSE HAEMOPOIETIC COLONY FORMING CELLS IN CULTURE (CFUc)

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Abstract
The kinetics of [mouse] CFUc [granulocytic progenitor cells] studied by hydroxyurea and endotoxin treatments indicate that this cell population is proliferating actively (some 50% in S [DNA synthesis during mitosis] phase). This fact implies a peculiar response to treatment with Bleomycin (BLM) [an antineoplastic drug], a drug which appears to be proliferation-dependent. The dose-response has a steep initial slope resulting in a low extrapolation number (n = 0.66; D37 [mean inactivation dose] = 960 .+-. 70 mg BLM/kg body wt). The steep initial slope is confirmed by split-dose experiments resulting in a potentiation effect by fractionation. Further evidence for the dependence of the action of this drug upon the proliferative state of the cell population is derived from time-response studies after single doses of BLM.