Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody.
- 24 May 1994
- journal article
- retracted article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (11) , 5075-5079
- https://doi.org/10.1073/pnas.91.11.5075
Abstract
Human immunodeficiency virus type 1 (HIV-1) has a complex life cycle, which has made it a difficult target for conventional therapeutic modalities. A single-chain antibody moiety, directed against the HIV-1 regulatory protein Rev, which rescues unspliced viral RNA from the nucleus of infected cells, has now been developed. This anti-Rev single-chain construct (SFv) consists of both light and heavy chain variable regions of anti-Rev monoclonal antibody, which, when expressed intracellularly within human cells, potently inhibits HIV-1 replication. This intracellular SFv molecule is demonstrated to specifically antagonize Rev function. Thus, intracellular SFv expression, against a retroviral regulatory protein, may be useful as a gene therapeutic approach to combat HIV-1 infections.Keywords
This publication has 21 references indexed in Scilit:
- Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody.Proceedings of the National Academy of Sciences, 1993
- Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.Proceedings of the National Academy of Sciences, 1992
- Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element.Molecular and Cellular Biology, 1991
- Ras C-terminal processing enzymes—New drug targets?Cell, 1991
- Man-made antibodiesNature, 1991
- Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replicationCell, 1990
- Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latencyCell, 1990
- Functional dissection of the HIV-1 Rev trans-activator—Derivation of a trans-dominant repressor of Rev functionCell, 1989
- Intracellular immunizationNature, 1988
- Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genesNature, 1988