INHIBITION OF HIGH AND LOW-MOLECULAR WEIGHT UROKINASE IN PLASMA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 55  (3) , 430-436
Abstract
The rates of inhibition of high MW (HMW) and low MW (LMW) urokinase (UK) incubated in [human] plasma or with purified antithrombin III (AT-III) were compared. Using a fibrinolytic assay system to determine residual biologic activity, polyacrylamide gel electrophoresis to demonstrate the formation of complexes and selective immunoprecipitation techniques to identify plasma inhibitors participating in the neutralization process, it was established that HMW-UK is inhibited more rapidly than LMW-UK, in plasma and with purified AT-III, heparin (3-10 U[units]/ml) slightly accelerates the neutralization process in both systems and in plasma, several inhibitors (.alpha.2-macroglobulin, .alpha.I-antitrypsin and antithrombin III) neutralize activity of HMW-UK and LMW-UK.