Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
- 1 January 2001
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 15 (1) , 30-35
- https://doi.org/10.1101/gad.851701
Abstract
The human 56-kD U2AF65-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.Keywords
This publication has 33 references indexed in Scilit:
- Multiple roles for the yeast SUB2/yUAP56 gene in splicingGenes & Development, 2001
- Yeast TAFII145 Required for Transcription of G1/S Cyclin Genes and Regulated by the Cellular Growth StateCell, 1997
- U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction.Genes & Development, 1997
- The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNAJournal of Biological Chemistry, 1996
- Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.Proceedings of the National Academy of Sciences, 1996
- CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.Genes & Development, 1996
- Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1994
- Requirements for U2 snRNP addition to yeast pre-mRNANucleic Acids Research, 1992
- An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.Genes & Development, 1991
- Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicingCell, 1989