Secondary mutation resistant to 7-ketocholesterol rescues a sterol metabolic defect in amphotericin B-resistant Chinese hamster cell line
- 1 November 1983
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 9 (6) , 659-672
- https://doi.org/10.1007/bf01539471
Abstract
Amphotericin B-resistant mutants isolated from Chinese hamster V79 cells (1) are defective in cholesterol synthesis and more sensitive to an oxygenated sterol analog, 7-ketocholesterol, than their parental cell line. We isolated 7-ketocholesterol-resistant mutants from an amphotericin B-resistant mutant, AMBR-1. The 7-ketocholesterol-resistant mutants had regained increased level of free cholesterol, and they showed somewhat similar dose-response curves to amphotericin B as that of V79. Sterol synthesis from acetate, but not from mevalonate, in 7-ketocholesterol-resistant clones was threefold higher than that of AMBR-1. 7-Ketocholesterol-resistant clone, unlike AMBR-1, could form colonies in the presence of lipoprotein-depleted serum. The results are discussed in terms of probable change in the sterol biosynthetic pathway by the different lesions.This publication has 30 references indexed in Scilit:
- Ultrastructural changes during the enhancement of cellular 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase in a Chinese hamster cell mutant resistant to compactin (ML 236B).Cell Structure and Function, 1983
- Complementary recessive 25-hydroxycholesterol-resistant somatic cell mutants - assay of 25-hydroxycholesterol binding activityJournal of Cellular Physiology, 1982
- Revertants of a Chinese hamster ovary cell mutant resistant to suppression by an analog of cholesterol: isolation and partial biochemical characterizationBiochemistry, 1982
- Isolation and preliminary characterization of bleomycin‐resistant mutants from Chinese hamster ovary cellsJournal of Cellular Physiology, 1981
- Amphotericin B resistance is recessive in Chinese hamster hybrid cellsJournal of Cellular Physiology, 1981
- Growth of amphotericin B-resistant hamster cell line requires exogenous cholesterolExperimental Cell Research, 1980
- Coated pits, coated vesicles, and receptor-mediated endocytosisNature, 1979
- Isolation and characterization of amphotericin B-resistant cell lines in Chinese hamster cellsCell, 1978
- Genetics of the LDL receptor: Evidence that the mutations affecting binding and internalization are allelicCell, 1977
- Isolation of a mammalian cell mutant resistant to 25-hydroxy cholesterolBiochemical and Biophysical Research Communications, 1977