Synthesis of theStreptomyces lividansmaltodextrin ABC transporter depends on the presence of the regulator MalR
Open Access
- 1 March 2001
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 196 (1) , 77-83
- https://doi.org/10.1111/j.1574-6968.2001.tb10544.x
Abstract
During growth with maltotriose or amylose, Streptomyces lividans and Streptomyces coelicolor A3(2) synthesize a maltodextrin uptake system with highest specificity for maltotriose. The transport activity is absent in mutants of S. coelicolor A3(2) lacking a functional MalE binding protein. Cloning and sequencing data suggest that the mal operon of S. coelicolor A3(2) corresponds to the one of S. lividans and that the deduced S. lividans Reg1 amino acid sequence is identical to that of MalR from S. coelicolor A3(2). It can be concluded that both strains have the same ABC transport system for maltodextrins. The S. lividans malR was cloned in Escherichia coli in frame with six histidine-encoding codons. The resulting, purified 6HisMalRSI was shown to bind to two motifs within the S. lividans malR–malE intergenic region and to dissociate in the presence of maltopentaose.Keywords
This publication has 19 references indexed in Scilit:
- Binding characteristics of CebR, the regulator of theceboperon required for cellobiose/cellotriose uptake inStreptomyces reticuliFEMS Microbiology Letters, 2000
- Molecular analysis of themalRgene ofClostridium butyricumNCIMB 7423, a member of the LacI-GalR family of repressor proteinsFEMS Microbiology Letters, 1998
- Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI-galR family of regulatory genesMolecular Microbiology, 1996
- Biochemical identification of a lipoprotein with maltose-binding activity in the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldariusResearch in Microbiology, 1996
- A Lipid‐Anchored Binding Protein is a Component of an ATP‐Dependent Cellobiose/Cellotriose‐Transport System from the Cellulose Degrader Streptomyces reticuliEuropean Journal of Biochemistry, 1996
- Catabolite repression mediated by the catabolite control protein CcpA in Staphylococcus xylosusMolecular Microbiology, 1996
- A cellulase/xylanase-negative mutant of Streptomyces lividans 1326 defective in cellobiose and xylobiose uptake is mutated in a gene encoding a protein homologous to ATP-binding proteinsMolecular Microbiology, 1995
- Crystal Structure of LacI Member, PurR, Bound to DNA: Minor Groove Binding by α HelicesScience, 1994
- Molecular characterization of malQ, the structural gene for the Escherichia coli enzyme amylomaltaseMolecular Microbiology, 1988
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985