Basophilic Lamellar Systems in the Crayfish Spermatocyte

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Abstract
Histo-chemical procedures for the demonstration of RNA have shown the presence of intensely basophilic bodies in the cytoplasm of spermato-cytes of the crayfish, Cambarus virilis. The staining of thick sections, cut alternately with thin sections for electron microscopy, has permitted identification of the basophilic bodies with two types of lamellar systems. One of these, a set of straight annulate lamellae, is restricted to meiotic prophase. The second type of lamellar systems has been found from late prophase to early spermatid stages. It consists of an ellipsoidal lamellar set which intersects a number of straight lamellae. Within the region of intersection, the ellipsoidal lamellae break up into an array of small tubules of about 150 A diameter. The term tubulate lamellar system was chosen to designate this type of lamellar complex. Small RNA-containing granules could not be detected in annulate lamella systems. While there are a few granules in the marginal regions of the tubulate lamellar system, their distribution cannot be responsible for the basophilia which is intense within all regions of the lamellar body.