Chick Embryo Extract, Muscle Trophic Factor and Chick and Horse Sera as Environments for Chick Myogenic Cell Growth

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Abstract
Chick myogenic cells grew in a medium composed of Eagle''s minimum essential medium (MEM), horse serum (HS) and 1 of the essential factors needed for myogenic cell growth (EFMG), i.e., chick embryo extract (EE), chick serum (CS) or the muscle trophic factor (MTF). They did not grow in the absence of the EFMG. In the absence of HS, they scarcely grew in a medium composed of MEM, and EE or MTF. They grew in a medium composed of MEM and CS; they grew much better in a medium composed of MEM, CS and HS. In the presence of 1 of the EFMG, the optimal HS concentration for growth varied depending on its lot. At higher HS concentrations, growth was suppressed. An inhibitory substance(s) for myogenic cell growth apparently was present in HS. The inhibitory effects can usually be minimized by diluting the serum with an artificial medium.