Biochemistry of the wood-rotting fungi. 9. Volatile metabolic products of Stereum subpileatum Berk. & Curt

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Abstract
S. subpileatum. a basidiomycete found growing in Persian-oak beer barrels and causing contamination of the beer, was cultured on a malt medium and on a glucose-Marmite-salts medium. The steam-volatile metabolic products, which were qualitatively the same on both media, were examined. Three metabolic products were isolated and identified: cinnamaldehyde, cinnamic acid and 5-methoxycoumarone. 5-Methoxycoumarone when treated with bromine in carbon disulphide afforded 2 crystalline products: colorless x-bromo-5-methoxycourmarone, m.p. 76[degree], and yellow 2:3:x-tribromo-5-methoxycoumaran, m.p. 80[degree]. 5-Methoxycoumarone is the product mainly responsible for the characteristic penetrating odor of the fungus. 5-Methoxycoumarone exhibited only weak antibacterial activity against Staphylococcus aureus and Escherichia coli.

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