The Reduction of 2,3,5-Triphenyltetrazolium Chloride by Penicillium chrysogenum
- 18 February 1949
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 109 (2825) , 169-170
- https://doi.org/10.1126/science.109.2825.169
Abstract
Some proof of the theory that penicillin is produced by a relatively inactive mold has been obtained by using the en-zymic reduction of 2,3,5-triphenyltetrazolium chloride (TTC) as an indicator of cell viability. P. chrysogenum Q176 was grown as described by Koffler and harvested. The pellets were washed, suspended in [image]/15 phosphate soln. and torn apart in a Waring Blendor. After blending, a buffer soln. of TTC was added to give a final concn. of 0.5%. Under these conditions, active mold cells reduce the dye to a deep red color in 20 min. at 30[degree]C. The ability of P. chrysogenum of different ages to reduce the TTC was observed as well as the yield of penicillin at the time of harvest. The older cells produced a greater yield of penicillin but showed less reduction of TTC.Keywords
This publication has 4 references indexed in Scilit:
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- Triphenyltetrazolium Chloride, A Valuable Reagent in Stain TechnologyStain Technology, 1948
- The Use of 2,3,5-Triphenyl-Tetrazoliumchloride as a Measure of Seed GerminabilityPlant Physiology, 1947
- Chemical Changes in Submerged Penicillin FermentationsJournal of Bacteriology, 1945