CHARACTERIZATION OF ANTIBODIES IN NORMAL HUMAN URINE*

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Abstract
Urines from persons immunized with tetanus toxoid or poliovirus vaccine contained low-molecular-weight antibodies specific for the immunizing antigens. Neutralizing activity to poliovirus was found in gamma-globulins with an Sw, 20 of 1.7. Precipitating antibody activity against tetanus toxoid was found in proteins with a molecular weight of 12,900 and Sw,20 of 0.92. The peptide patterns after tryptic digestion of urinary gamma-globulins differed from those after tryptic digestion of serum 7S gamma-globulins. Immunochemical and isotopic studies indicated that the low-molecular-weight gamma-globulins are heterogeneous and may have multiple origins.