Reversed-Phase High Performance Liquid Chromatographic Separation of Small Molecules on Nonporous C18 Silica Gel Column

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Abstract
Reversed-phase high-performance liquid chromatographic (RP HPLC) separation of small molecules on a nonporous octadecylsily (C18) spherical silica gel was compared with the separation or. conventional porous packings. Various alkylbenzenes were separated on three different types, nonporous, macroporous (pore diameter; 30 nm), and microporous (pore diameter; 10 nm), C18 bonded silica columns under both isocratic and gradient elution. conditions. Under the isocratic conditions, column efficiency of nonporous silica was inferior to that of others. In case of the gradient elution, the nonporous silica column gave the most. favorable separation in a short time. Fast separation of srall molecules on the nonporous column could be performed by using a conventional HPLC system with a steep gradient condition. because the void volume of the nonporous packing was extremety smaller than the porous ones. Various series compounds such as fatty acid derivatives, phenylthiohydantoin (PTH) amino acids, and vitamins were well separated within 1 or 2 min on the C18 nonporous silica column under steep gradient conditions.

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