T cell receptor diversity and activation markers in the Vδ1 subset of rheumatoid synovial fluid and peripheral blood T lymphocytes

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Abstract
In the present study we have characterized the γ/δ T cell receptor (TcR) population in synovial fluid (SF) and peripheral blood (PB) of patients with chronic inflammatory arthritis. By double staining we have shown that (a) synovial Vδ1+ cells have a high expression of activation markers CD45R0 (“memory cells”) and HLA-DR as compared to PB, indicating a preactivated population of Vδ1-carrying T cells in vivo and (b) interleukin 2-induced expansion of synovial cells yields a high proportion of γ/δ in most samples expressing predominantly the Vδ1 TcR. Junctional sequence analysis of the TcR δ chain from interleukin 2-expanded PB cell lines demonstrated a polyclonal Vδ1 population in three out of three samples. In SF cell lines three out of four samples were polyclonally expanded. In SF from one patient, however, a limited repertoire of expressed Vδ1 genes was found. Altogether, our data demonstrate the presence of preactivated Vδ1-expressing cells in the synovial compartment. This Vδ1 population is predominantly polyclonal, except in one patient where oligoclonally expanded Vδ1 cells were detected.