A simple DNA-dependent fluorescence enhancement assay for cell number.
Open Access
- 1 February 1981
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 29 (2) , 326-328
- https://doi.org/10.1177/29.2.7252122
Abstract
A DNA-dependent fluorescence enhancement assay using the dye Hoechst 33258 was developed to quantitate cell number in crude detergent lysates of cells. The assay, which uses a simple filter fluorometer, is sensitive enough to measure 1 x 10(4) cells without interference from mercaptans, culture media, and protein solutions. They assay can be done with freeze-thawed lysates. Fluorescence intensity values are linearly related to cell number in the range of 1 x 10(4) to 2.5 x 10(5) cells for L-929, BHK, and CHO-Kl cell lines.This publication has 5 references indexed in Scilit:
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