Hybridization and dissociation rates of phosphodiester or modified oligodeoxynucleotides with RNA at nearphysiological conditions.
Open Access
- 1 January 1991
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (9) , 2463-2470
- https://doi.org/10.1093/nar/19.9.2463
Abstract
We have used RNase H to study both the rates of ollgonucleotide hybridisation and dissociation at nearphysiological conditions. We have studied the Effects of oligonucleotide length, mismatch, and chemical modifications on oligonucleotide association and dissociation with RNA. Dissociation results were compared with standard thermal melting curves to compare relative stabilities evaluated by the two techniques. Although generally the two techniques correlate for the compounds evaluated, we found several instances where the thermal melting curves failed to reflect the relative stability of different oligonucleotldes at 37°C using near-physiological conditions. This study suggests that direct measurement of hybridization and dissociation of an ollgomer with RNA more accurately assesses the complicated kinetic scheme at 37°C using nearphyslologlcal conditions than thermal melting curves would predict.Keywords
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