Intra-specific variation withinEimeria tenella detected by the random amplification of polymorphic DNA
- 1 May 1994
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 80 (4) , 346-351
- https://doi.org/10.1007/bf02351878
Abstract
The random amplification of polymorphic DNA (RAPD method), was used to derive fragments of DNA of the coccidial parasiteEimeria tenella for use both as genetic markers and as an aid for the discrimination of different wild-type and attenuated populations. Of 41 arbitrarily chosen 9-to 27-mer primers, 24 yielded useful arrays of fragments following low-stringency annealing conditions and the resultant profiles were generally very reproducible. One non-variant fragment of 2 kb hybridised to a single chromosome (number 12) and four variant fragments were identified. These results strongly suggest that the RAPD method may be an extremely useful tool for studies on various aspects of the genetic organisation of coccidial parasites.Keywords
This publication has 20 references indexed in Scilit:
- Coccidial parasites from the chicken: discrimination of different populations of Eimeria tenella by DNA hybridisationResearch in Veterinary Science, 1994
- Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markersMolecular and Biochemical Parasitology, 1993
- Inter- and intra-species differentiation of trypanosomes by genomic fingerprinting with arbitrary primersMolecular and Biochemical Parasitology, 1993
- The random amplification of polymorphic DNA allows the identification of strains and species of schistosomeMolecular and Biochemical Parasitology, 1993
- A molecular karyotype of Eimeria tenella as revealed by contour-clamped homogeneous electric field gel electrophoresisMolecular and Biochemical Parasitology, 1990
- A comparative study of two lines ofEimeria tenellaattenuated either by selection for precocious development in the chicken or by growth in chicken embryosAvian Pathology, 1986
- Purification of Eimeria Sporozoites by DE‐52 Anion Exchange ChromatographyThe Journal of Protozoology, 1984
- Some observations on the sexual differentiation of Eimeria tenella using single sporozoite infections in chicken embryosParasitology, 1976
- Comparative In Vitro Development of Precocious and Normal Strains of Eimeria tenella (Coccidia)The Journal of Protozoology, 1976
- Enzyme variation in Eimeria species of the chickenParasitology, 1975