Response of CO2-Deficient Human Cells in vitro to Normal Cell Extracts.
- 1 December 1958
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 99 (3) , 599-601
- https://doi.org/10.3181/00379727-99-24432
Abstract
Human cells of HeLa and conjunctival (Chang) strains fail to multiply in absence of CO2, but net multiplication can be restored by addition of normal cell extract. Such extracts have no effect on multiplication of normal cells. On the other hand, extracts of CO2-deficient cells have an adverse effect on deficient cells. Although net multiplication was completely arrested in the absence of CO2, conversion of acetate-1-C14 to C14O2 was at least normal, indicating general cell metabolism was still high. Implications of the results to regulation of cell multiplication are discussed.Keywords
This publication has 5 references indexed in Scilit:
- Bicarbonate as an essential for human cells in vitroArchives of Biochemistry and Biophysics, 1958
- The salt requirements of mammalian cells in tissue cultureArchives of Biochemistry and Biophysics, 1956
- A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORSProceedings of the National Academy of Sciences, 1955
- Studies on the metabolic interplay of fatty acids, succinate and electrolytesArchives of Biochemistry and Biophysics, 1955
- The role of bicarbonate for outgrowth of chick heart fibroblasts in vitroJournal of Experimental Zoology, 1954