Deletion analysis of the c-Ha-ras oncogene promoter
- 13 July 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 219 (1) , 70-74
- https://doi.org/10.1016/0014-5793(87)81192-4
Abstract
Using transient assays of chloramphenicol acetyltransferase expression in CV-1 cells, we have localized the human c-Ha-ras oncogene promoter to a 550 bp fragment located about 1 kb upstream from the ras coding sequence. Deletion analysis has revealed that a 100 nucleotide region 200 base pairs upstream of the putative initiation sites for transcription is essential for high levels of expression. Within this sequence lie two Spl binding sites and a consensus CCAAT box.Keywords
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