Intracellular and Extracellular Particles in Tissue Cultures Inoculated With Parotid-Tumor Agent (Polyoma Virus)

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Abstract
Cell cultures of murine lymphoma strain P388 D1 were inoculated with parotid-tumor agent (polyoma virus) and examined with the electron microscope after the appearance of cytopathogenic effect. Intranuclear, intracytoplasmic, and extracellular spherical particles of relatively uniform appearance, measuring approximately 27 to 35 mµ in diameter, were found. Similar particles did not occur in uninfected control cultures. Newborn C3Hf/BiLw mice inoculated with the infected cultures developed salivary-gland tumors and multiple tumors of other types associated with this agent, whereas mice receiving control cultures did not. A second type of particle similar to that described by Dmochowski and Grey in murine and human leukemias was found both in test and control cultures. This type of particle was not associated with leukemia in the present study.

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