New directions of miniaturization within the proteomics research area
Open Access
- 6 November 2003
- journal article
- review article
- Published by Wiley in Electrophoresis
- Vol. 24 (21) , 3521-3532
- https://doi.org/10.1002/elps.200305666
Abstract
An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel‐ and liquid‐phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low‐abundant protein expressions where assay miniaturization and integrated sample processing plays a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.Keywords
This publication has 48 references indexed in Scilit:
- Nanocapillary liquid chromatography interfaced to tandem matrix‐assisted laser desorption/ionization and electrospray ionization‐mass spectrometry: Mapping the nuclear proteome of human fibroblastsElectrophoresis, 2003
- An accurate mass tag strategy for quantitative and high-throughput proteome measurementsProteomics, 2002
- Miniaturisation is mandatory unravelling the human proteomeProteomics, 2002
- On-chip microextraction for proteomic sample preparation of in-gel digestsProteomics, 2002
- Improved chip design for integrated solid-phase microextraction in on-line proteomic sample preparationProteomics, 2002
- Two-dimensional gel electrophoresis in proteomics: Old, old fashioned, but it still climbs up the mountainsProteomics, 2002
- Simplified sample preparation method for protein identification by matrix-assisted laser desorption/ionization mass spectrometry: In-gel digestion on the probe surfaceProteomics, 2001
- Differential stable isotope labeling of peptides for quantitation and de novo sequence derivationRapid Communications in Mass Spectrometry, 2001
- Back to the future: The human protein index (HPI) and the agenda for post-proteomic biologyProteomics, 2001
- Capillary liquid chromatography interfaced to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using an on-line coupled piezoelectric flow-through microdispenserJournal of Mass Spectrometry, 2000