Rat Hepatocyte-Mediated Mutagenesis of Human Cells by Carcinogenic Polycyclic Aromatic Hydrocarbons but Not Organochlorine Pesticides

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Abstract
A [rat] hepatocyte primary culture-mediated human cell hypoxanthine-guanine phosphoribosyl transferase mutagenesis assay was established. This assay provides an in vitro system that combines a realistic activation capability and an endpoint of clear biological significance in a target cell line relevant to human risk. The genotoxicity of 7,12-dimethylbenz[a]anthracene and benzo[a]pyrene were demonstrated in this assay. In the same assay, DDT, chlordane, and mirex were not genotoxic. An epigenetic mechanism for the carcinogenicity of these organochlorine pesticides is suggested.