Requirement of Initiation Factor 3 in the Initiation of Polypeptide Synthesis with N -Acetylphenylalanyl-tRNA
- 1 March 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (3) , 774-778
- https://doi.org/10.1073/pnas.71.3.774
Abstract
Initiation factor 3 is required, along with initiation factors 1 and 2, for the incorporation of N-acetylphenylalanine into polypeptides and the formation of N-acetylphenylalanylpuromycin. Initiation factor 3 also strongly stimulates the binding of N-acetylphenylalanyl transfer RNA to isolated 30S ribosomal subunits. Phosphocellulose fractions of initiation factor 3 were found to catalyze N-acetylphenylalanine incorporation differentially with different synthetic messenger RNAs not containing any codons for N-formylmethionine. The results suggest that ribosomes recognize the initiator codon only through the initiator transfer RNA.Keywords
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